ChIP

Chromatin immunoprecipitation (ChIP) studies the interactions between DNA and proteins, providing a more authentic and complete reflection of regulatory proteins bound to DNA sequences. After sample cross-linking, DNA fragments with lengths concentrated between 100-750 bp are obtained through chromatin shearing, which can be used for next-generation sequencing to obtain information of DNA and protein interactions.

BoFU-80 concentrates acoustic energy on the sample to be processed through a high-frequency focused ultrasound system efficiently. BoFU-80 could achieve efficient, highly repetitive, and low protein damage in chromatin shearing. After cross-linking and shearing of bacteria or cell samples, using agarose gel ealctrophoresis to detect. The sample numbers for each lane labeled as Marker, sample 1, fragmented sample 1, sample 2, fragmented sample 2, sample 3, fragmented sample 3, sample 4, fragmented sample 4 sequentially . It can be seen that the DNA integrity is great from lanes 1, 3, 5, and 7. And from lanes 2, 4, 6, and 8, it can be seen that after chromatin shearing the length of DNA fragments is concentrated between 100-750 bp, and there is high repeatability between samples.