DNA Library Prep Kit for Illumina & MGI
LL Universal DirectFast DNA Library Prep Kit is a next-generation sequencing enzyme-based library kit developed for Illumina and MGI sequencing platforms. It contains three major blocks of premixed enzymes required for DNA fragmentation/end repair/A-tailing, Adapter ligation and library enrichment in library preparation. Controlled reaction time allows sample DNA from different sources, such as 0.1 ng-1 μg genomic DNA, PCR amplification products, FFPE, etc., to be made into small fragments, avoiding cumbersome ultrasound processes and instrument dependence.
DNA Library Prep Kit for Illumina & MGI Features
- Suitable for FFPE samples: Optimizing and upgrading FFPE samples and low-input samples improves the quality and yield of libraries.
- Automable: The performance can meet the needs of database creation automation.
- Complete reagents: comes with purification magnetic beads, no need to purchase them separately.
- Amplification uniformity: High-fidelity enzymes perform PCR enrichment amplification, which minimizes amplification bias and improves the uniformity of sequence coverage.
Description of Biomagnetic Separators
LL Universal DirectFast DNA Library Prep Kit is a next-generation sequencing enzyme-based library kit developed for Illumina and MGI sequencing platforms. It contains three major blocks of premixed enzymes required for DNA fragmentation/end repair/A-tailing, Adapter ligation and library enrichment in library preparation. Controlled reaction time allows sample DNA from different sources, such as 0.1 ng-1 μg genomic DNA, PCR amplification products, FFPE, etc., to be made into small fragments, avoiding cumbersome ultrasound processes and instrument dependence. This kit simplifies the purification step, shortens the setup time, uses high-fidelity DNA polymerase for library enrichment, and performs PCR amplification without preference to ensure the accuracy of sequencing results.
It is suitable for library preparation of different species such as humans, animals, plants, microorganisms, cDNA, long amplicons, plasmids, etc. The resulting library is suitable for various platforms of Illumina and MGI
Advantages of DNA Library Prep Kit for Illumina & MGI
1.Easy to operate and Precise enzyme shearing
[Experiment 1] Genomic DNA shearing, termination of repair, is completed in one step, and different fragment sizes can be adjusted through the interruption time and are not affected by the amount of participation, as shown in the figure below:
Figure 1 Fragment size under different interruption times
2.Suitable for FFPE samples
[Experiment 2] For FFPE samples with different degradation degrees, use Longlight enzyme- containing DNA library preparation kit to prepare targeted libraries, as shown in the figure below:
| Sample type | Quality | Input (ng) | Shearing time (min) | Amplification cycle | Concentration
(ng/μL) |
Volume (μL) |
| FFPE DNA | A | 100 | 20 | 6 | 65.2 | 30 |
| FFPE DNA | A | 100 | 20 | 7 | 60.7 | 30 |
| FFPE DNA | B | 100 | 15 | 7 | 64.1 | 30 |
| FFPE DNA | C | 100 | 12 | 7 | 55.6 | 30 |
Figure 2 Library preparation results of FFPE samples with different degradation degrees
Experimental results show that FFPE samples with different degradation degrees can obtain appropriate fragment sizes and higher library output after library construction.
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